A highly-available and scalable microservice architecture for access management

Access management is a key aspect of providing secure services and applications in information technology. Ensuring secure access is particularly challenging in a cloud environment wherein resources are scaled dynamically. In fact keeping track of dynamic cloud instances and administering access to them requires careful coordination and mechanisms to ensure reliable operations. PrivX is a commercial offering from SSH Communications and Security Oyj that automatically scans and keeps track of the cloud instances and manages access to them. PrivX is currently built on the microservices approach, wherein the application is structured as a collection of loosely coupled services. However, PrivX requires external modules and with specific capabilities to ensure high availability. Moreover, complex scripts are required to monitor the whole system. The goal of this thesis is to make PrivX highly-available and scalable by using a container orchestration framework. To this end, we first conduct a detailed study of mostly widely used container orchestration frameworks: Kubernetes, Docker Swarm and Nomad. We then select Kubernetes based on a feature evaluation relevant to the considered scenario. We package the individual components of PrivX, including its database, into Docker containers and deploy them on a Kubernetes cluster. We also build a prototype system to demonstrate how microservices can be managed on a Kubernetes cluster. Additionally, an auto scaling tool is created to scale specific services based on predefined rules. Finally, we evaluate the service recovery time for each of the services in PrivX, both in the RPM deployment model and the prototype Kubernetes deployment model. We find that there is no significant difference in service recovery time between the two models. However, Kubernetes ensured high availability of the services. We find that Kubernetes is the preferred mode for deploying PrivX and it makes PrivX highly available and scalable

Paclitaxel resistance in untransformed human mammary epithelial cells is associated with an aneuploidy-prone phenotype

Despite its increasing clinical use, almost no data are currently available about paclitaxel effects on non-cancerous mammary epithelial cells. We have previously established paclitaxel-resistant sub-cell lines (paclitaxel-surviving populations, PSPs; n=20), and sensitive controls (control clones, CCs; n=10), from the untransformed human mammary epithelial cell line HME1. In this study, we aimed to establish whether paclitaxel resistance was associated with a modified sensitivity to paclitaxel-induced aneuploidy. For this purpose, we analysed basal and paclitaxel-induced chromosome missegregation, apoptosis and aberrant spindle multipolarisation as well as microtubular network composition for each subline. PSP sublines showed higher basal and paclitaxel-induced chromosome missegregation than the CC sublines. This phenomenon was associated with resistance to paclitaxel-induced apoptosis. No significant difference in paclitaxel-induced spindle pole abnormalities between CC and PSP sublines was found. Besides, we showed that a majority of PSPs display a constitutively disrupted microtubular network composition due to aberrant tubulin expression and post-translational modifications. These results clearly indicate that paclitaxel resistance in untransformed human mammary epithelial cells is related to an increased susceptibility to acquire aneuploidy in response to this agent. The consequences of these paclitaxel-associated alterations could be deleterious as they can potentially trigger tumorigenesis

A randomised comparative evaluation of supraclavicular and infraclavicular approaches to brachial plexus block for upper limb surgeries using both ultrasound and nerve stimulator

Background and Aims: The supraclavicular and infraclavicular brachial plexus blocks have a similar distribution of anaesthesia, and both can be used effectively for surgeries of the upper limb. This study aimed to compare the supraclavicular and infraclavicular approaches of brachial plexus blocks, guided by ultrasound and neurostimulation. Methods: Sixty adult patients scheduled for elective upper limb surgery of the elbow and/or below were randomly divided into two groups: infraclavicular Group (I) and supraclavicular Group (S). All the blocks were performed with the aid of ultrasound-guided nerve stimulator confirmation. The two groups were compared with respect to block performance time, onset of sensory and motor blockade, readiness for surgery, success rate and complications. The statistical analysis was performed with Student t-test and Chi-square test. Results: The block performance time for the infraclavicular group was 9.57 ± 3.19 min, whereas for supraclavicular group, it was 11.53 ± 2.90 min with similar success rates (93.3%). Onset of sensory blockade was achieved earlier (6.43 ± 2.61 min) in Group I than Group S (8.45 ± 2.87 min, P = 0.006). The onset of motor blockade was similar in Group I (7.32 ± 2.90 min) and Group S (8.68 ± 3.50 min, P = 0.121). The patient satisfaction was similar in both the groups. One patient had a pneumothorax, three patients developed Horner syndrome and another had clinically symptomatic diaphragmatic paresis in Group S. Conclusion: The infraclavicular block is more rapidly executed compared to supraclavicular block with similar success rates and fewer complications in the presence of ultrasound and nerve stimulator and hence should be preferred

Phytolith analysis reveals the intensity of past land use change in the Western Ghats biodiversity hotspot

This paper presents a study of phytoliths (opal silica bodies from plants) from sediment sequences obtained from two tropical forest patches in the Western Ghats of India: a sacred grove (sequence covers last 550 cal BP) and a forest patch in a plantation (sequence covers last 7500 cal BP). The sites are located at mid-elevation (c. 650–1400 m above sea level) in a mosaic landscape showing anthropogenic open habitats and plantation as well as some evergreen forests. The aim of this paper is to evaluate the landscape composition of grassland and forest over time in the region, grassland being invariably shaped by anthropogenic activities, particularly fire for cultivation. In particular, we compare the phytolith record with the previously published pollen record from the same cores in order to improve our understanding of grass diversity and abundance over time. We identified and classified phytoliths into 34 morphotypes from five taxonomic groups: Poaceae (grasses), Cyperaceae (sedges), Arecaceae (palms), Pteridopsida (ferns) and woody dicotyledons (broad-leaved trees and shrubs). We also calculated the humidity–aridity index (Iph). First, our results show that grasses are the most represented phytolith types in both sites, followed by broad-leaved trees and shrubs, palms, sedges, and ferns. Second, the highly variable climatic index Iph over the last 1000 years suggest that changes in phytolith percentage (e.g. broad-leaved trees) might be caused by human agro-pastoral activities, such as clearing through fires and irrigation. Prior to these human activities, the phytolith signal for early Holocene climate is congruent with the existing literature. Finally, this study compares new phytolith results with previous pollen data from the same sites. We find good agreement between these two botanical proxies throughout, thus validating our findings. We provide important evidence regarding the history of environmental change due to anthropogenic activities in the Western Ghats. This has important implications because it provides insights into how tropical forest will respond to increased intensity of human activities

Evidence of Significant Central Fatigue in Patients with Cancer-Related Fatigue during Repetitive Elbow Flexions till Perceived Exhaustion

<div><p>Objective</p><p>To investigate whether fatigue induced by an intermittent motor task in patients with cancer-related fatigue (CRF) is more central or peripheral.</p><p>Methods</p><p>Ten patients with CRF who were off chemo and radiation therapies and 14 age-matched healthy controls were enrolled. Participants completed a Brief Fatigue Inventory (BFI) and performed a fatigue task consisting of intermittent elbow-flexion contractions at submaximal (40% maximal voluntary contraction) intensity till self-perceived exhaustion. Twitch force was elicited by an electrical stimulation applied to the biceps brachii muscle. The relative degree of peripheral (muscle) vs. central contribution to fatigue induced by the intermittent motor task (IMT) was assessed using twitch force ratio (TF_ratio) defined as post IMT twitch force to pre IMT twitch force. The total number of trials (intermittent contractions) and total duration of all trials performed by each subject were also quantified.</p><p>Results</p><p>BFI scores were higher (p<0.001) in CRF than controls, indicating greater feeling of fatigue in CRF patients than controls. A significantly smaller number of trials and shorter total duration of the trials (p<0.05) were observed in CRF than control participants. The TF_ratio (0.81±0.05) in CRF was higher (p<0.05) compared with that of controls (0.62±0.05), suggesting CRF patients experienced a significantly lower degree of muscle (peripheral) fatigue at the time of perceived exhaustion.</p><p>Conclusion</p><p>Consistent with prior findings for fatigue under submaximal sustained contraction, our results indicate that motor fatigue in CRF is more of central than peripheral origin during IMT. Significant central fatigue in CRF patients limits their ability to prolong motor performance.</p></div

Mean duration (A), normalized force amplitude (B), normalized force area (C), ascending slope (D) and descending slope (E) over 20 trials at the beginning (Beg), middle (Mid) and end (End) blocks of the IMT representing mild, moderate and severe fatigue stages, respectively in controls (open circles) and CRF patients (filled circles).

<p>CRF  =  cancer-related fatigue, CTL  =  control.</p

Normalized (%MVC) pre- and post-IMT twitch forces in CRF patients and controls.

<p>CRF  =  cancer-related fatigue, CTL  =  control.</p